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Residues Q59 and N61 ended up mutated together into a set of histidine residues to create an HxH motif that is definitely analogous to that found in NagA from B. subtilis
At the moment one.5 mL of acetic anhydride was additional and allowed to respond at home temperature right away. Right after elimination with the solvent, the residue was dissolved in a hundred mL of chloroform and washed with dilute HCl, saturated NaHCO3 and after that dried over Na2SO4. The product, diphenyl N
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